Bacteriological valuations of some powdered infant milk and antibiotic resistance estimation

Authors

  • Sameira S. Swilaiman Department of Basic Sciences, College of Dentistry, Hawler Medical University, Kurdistan Region, Iraq.
  • Lawin A. Omer Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq.
  • Suhaila N. Darogha Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq.

DOI:

https://doi.org/10.15218/edj.2022.6

Keywords:

Powdered infant milk, Stain, Antibiotic, Staphylococci, Lactobacillus

Abstract

Background and objectives: Milk is one of the widely consumed products in the world, highly susceptible to contamination by microorganisms and it is also a suitable medium for the rapid growth and multiplication of bacteria at favorable temperatures, infant formula (baby milk) contain purified cow's milk whey and casein as a protein source, the high nutrient contents of infant formula provide a good growth medium for bacterial pathogens. This study aimed to evaluate the prevalence of bacterial contamination in powder milk sold in local market of Erbil city, and to determine their antibiotic susceptibility .
Method: In a total of 12 samples of commercial dried milk products (infant powdered milk) used by consumers in Erbil state, biochemical and molecular identification were used for identification. A region of 16S rRNA gene were amplified by PCR to allow species identification, and the sensitivity of isolates to eight antibiotics was studied by using standard disc diffusion method
Results: Eight samples of infant powdered milk (N = 12) were contaminated with (62.5%) Staphylococcus sp and (37.5%) Lactobacillus sp . In addition, Staphylococcus sp. resistance against AMC and E was between (72-69%) and the lowest resistance was against AMK and TE (25-28%). Lactobacillus species resistance was most frequently observed to E and AMC (88-83%), and lowest resistance was against CIP,TE ( 25-30%)
Conclusion : The current study found that infant powdered milk used by consumers in Erbil state were contaminated with Staphylococcus sp. and Lactobacillus , these isolates exhibited multiple antimicrobial resistance. No significant differences were found between isolates against antibiotic resistances.

References

Ali A, Akhtar N, Bashir U, Hafeez R, Haider M. Morphological and biochemical characterization of bacteria isolated from milk products. Biologia (Pakistan). 2015;61:271–7.

Afroz h H, Sultana F, Fakruddin M, Khan M, Uddin Z, Datta S. Isolation of escherichia coli and staphylococcus aureus from full cream powder milk sold under market conditions at Dhaka, Bangladesh and their antibiotic susceptibility. Journal of Advanced Scientific Research. 2013;4(3):27–31.

Shadlia-Matug M, Aidoo K, Candlish A, Elgerbi A. Evaluation of some antibiotics against pathogenic bacteria isolated from infant foods in North Africa. Open Food Science Journal. 2008;2:95–101.

Yacoub SS, Shamsia SM, Awad SA, Ziena HM, Safwat NM. Characterization of aerobic sporeforming bacteria isolated from raw milk, skim milk powder and UHT milk. Alexandria Science Exchange Journal. 2017;38(1):99–111.

Palilu PT, Budiarso TY. Isolation and identification of staphylococcus sp. in powdered infant milk. AIP Conference Proceedings. 2017;1844:020016.

Ahmed M, Hafez E, Mona A, Abdelrrassoul HA, Mabrouk Y .Detection of baby milk powder contamination by microorganisms. World Applied Sciences Journal. 2014;30:93–8.

Power DA, Johnson JA. 2011. difco™ & bbl™ manual.

Abdalla AM, Khalid AA, Mohammed SO. Detection of bacterial contamination in adult and infant powdered milk consumed in Khartoum state. World Applied Sciences Journal. 2014;30:93–8.

Thornsberry C. NCCLS standards for antimicrobial susceptibility tests. Laboratory Medicine. 2016;14:549–53.

Benda P, vyletĕlová M. Staphylococcus aureus in bulk milk samples. Veterinarni Medicina. 1996;40: 221–6.

Michanie S, Bryan FI, Alvarez P. Olivo AB. Critical control points for foods prepared in households in which babies had salmonellosis. International Journal of Food Microbiology. 1987;5:337–54.

Forsythe SJ. Enterobacter sakazakii and other bacteria in powdered infant milk formula. Maternal & Child Nutrition. 2005;1:44–50.

Reves F J, Bastias HJ, Gutierrez RM, Rodriguez LM. Prevalence of Bacillus cereus in dried milk products used by Chilean School Feeding Program. J Food Microbiol. 2007;24:1–6.

Wang X, Wang , Guo G, Usman T, Ao D, Tan X, Zhang Y. Yu Y. Antimicrobial resistance and toxin gene profiles of s taphylococcus aureus strains from holstein milk. Letters in Applied Microbiology. 2014;58: 527-34.

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Published

2022-07-30

How to Cite

1.
Swilaiman SS, A. Omer L, Darogha SN. Bacteriological valuations of some powdered infant milk and antibiotic resistance estimation. EDJ [Internet]. 2022 Jul. 30 [cited 2022 Aug. 10];5(1):44-9. Available from: https://edj.hmu.edu.krd/index.php/journal/article/view/158

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Original Articles