Evaluation of the penetration depth of Guttaflow bioseal and Bioceramic sealers using scanning electron microscope (in vitro study).

Authors

  • Razawa Kareem Saeed Department of Conservative Dentistry, College of Dentistry, Hawler Medical University, Erbil, Iraqi Kurdistan Region.

DOI:

https://doi.org/10.15218/edj.2023.26

Keywords:

Penetration depth, Guttaflow bioseal, Bioceramic Sealer, Scanning Electron Microscope

Abstract

Background and objective: Three- dimensional obturation of the canal is mandatory in root
canal treatment to decrease failure rate, the major cause of root canal reinfection after obturation
is insufficient obturation of the canal which later leads to a periapical lesion and reinfection.This
study aimed to evaluate the penetration depth of Guttaflow bioseal and Bioceramic sealer.
Methods: Twenty extracted single-rooted human teeth (mandibular first premolar) were used in
this study, teeth were decornated leaving 10 mm root length. The chemico-mechanical preparation of the samples was done by using a protaper universal system till size F1 and then samples
were divided into 2 groups (n = 10), according to the filling material in the first group Guttaflow
bioseal used while in the second group Onefil bioceramic sealer used the sealers introduced into
canals according to manufacturer instructions and then obturated with gutta-percha size F1, single condensation technique used and finally samples were cross-sectioned at 3 and 7 mm, sealers penetration depth was measured by using scanning electron microscope.
Result: Independent sample t-test shows that the difference between the penetration depth of
guttaflow bioseal and bioceramic sealers are non-significant while the ANOVA test shows that
the difference between the three sections of the root is highly significant for both sealer, coronal
section shows the best result for Guttaflow bioseal while middle section for Bioceramic shows
the best result of penetration.
Conclusion: There are no difference in sealer penetration of both sealers Guttaflow bioseal and
Onefil bioceramic, while regarding sealer penetration in each sections of root for Guttaflow bioseal in coronal section shows a best penetration, for Onefil bioceramic middle section shows a best penetration.

References

Daniela M, Gustavo S, Fabio LC, Villela B, Idomeo B. In-vitro assessment of the obturation capacity, adjustment and compaction of gutta-percha in the root channel framework utilizing distinctive filling systems. Acta Odontol Lantinoam. 2008; 21:3–9.

Ingle JI, Baumgartner JC, 2008. Ingle's endodontics PMPH: USA.

Adanir N, Cobankara FK, Belli S. Sealing properties of different resin‐based root canal sealers.

In Journal of Biomedical Materials Re-Search Part B: Applied Biomaterials: An Official Journal of the Society for Biomaterials, The Japanese

Society for Biomaterials, and The Australia Society for Biomaterials and the Korean Society forBiomaterials, 2006;77 (1), pp.1-4.

Aptekar A, Ginnan K. Comparative analysis of micro leakage and seal for 2obturation materials: Resilon /Epiphany and gutta-percha. J Can Dent Assoc 2006; 72:245.

Ravanshad S, Khayat A. An In-vitro Evaluation of Apical Seal Ability of Thermafil Obturation Versus Lateral Condensation. J Dent TUMS 2004; 1:48-55.

Williams C, Loushine RJ, Weller RN, Pashley DH, Tay FR, 2006. A comparison of cohesive strength stiffness of Resilon and gutta-percha. Journal of Endodontics 2006; 32 (6) pp. 553-555.

Nikhil V, Singh R. Confocal laser scanning microscopic investigation of ultrasonic, sonic, and rotary sealer placement techniques. Journal of Conservative Dentistry 2013; 16(4), p.294.

Robertson D, Leeb IJ, McKee M, Brewer E. A clearing technique for the study of root canal

systems. J Endod1980;6: 421-424.7.Coltene,GuttaFlow2,bioseal,https:japan.

coltene.com/products/endodontics/obturationamp- sealing-materials /roeko-sealers/ roekoguttaflowR-bioseal/

Keeneth K and Deenis B. Anew day has dawned: the increased use of bioceramics in endodontics. 2009 the increased use of bioceramics in endodontics Dentaltow vol. 10, pp. 39–43, 2009.

Ginebra MP, Fernández E, De Maeyer EA, Verbeeck RM, Boltong MG, Ginebra J. Setting reaction and hardening of an apatitic calcium phosphate cement.1997; J of Dental Research, vol. 76, no. 4, pp. 905–912.

Cherng AM, Chow LC, Takagi S. In vitro evaluation of a calcium phosphate cement root canal filler/sealer. Journal of Endodontics.2001; vol. 27, no. 10, pp.613–615.

Manu R, Gursandeep Kaur S, Tamanpreet Kaur, Mohd. A, Gayatri G. New Self Curing Root Canal Filling Material: Gutta flow. journal of medical and advanced dental science. 2014; vol2, issue 4.

Verissimo DM, do Vale MS. Methodologies for assessment of apical and coronal leakage of endodontic filling materials: A critical review. J Oral Sci 2006; 48:93-8.

Cohen S, Burns RC. Pathways of Pulp.8th ed. United States: Mosby; 2002.

Punia SK, Nadig P, Punia V. An in vitro assessment of apical micro leakage in root canals obtu-rated with guttaflow, resilon, thermafil and lateral condensation: A stereomicroscopic study. J Conserv Dent 2011; 14:173-7.

Nevares G., Xavier F., Gominho L., et al. Apical extrusion of debris produced during continuous rotating and reciprocating motion. The Scientific World Journal. 2015; 2015:5. doi: 10.1155/2015/267264.267264

Bronnec F., Bouillaguet S., Machtou P. Ex vivo assessment of irrigant penetration and renewal during the cleaning and shaping of root canals: a digital subtraction radiographic study. International Endodontic Journal. 2010;43(4):275–282. doi: 10.1111/j.1365-2591.2009.01677.x. [PubMed] [CrossRef] [Google Scholar]

Fleming C. H., Litaker M. S., Alley L. W., Eleazer P. D. Comparison of classic endodontic techniques versus contemporary techniques on endodontic treatment success. JournalofEndodontics. 2010;36(3):414–418. doi: 10.1016/j.joen.2009. [PubMed] [CrossRef] [Google Scholar]

Hwang Y.-H., Bae K.-S., Baek S.-H., et al. Shaping ability of the conventional nickel titanium and reciprocating nickel-titanium file systems: a comparative study using micro-computed tomography. Journal of Endodontics. 2014;40(8):1186–1189. doi:10.1016/j.joen.2013.12.032

Valera M. C., Cardoso F. G. D. R., Chung A., et al. Comparison of different irrigants in the removal of endotoxins and cultivable microorganisms from infected root canals. The Scientific World Journal. 2015;2015: 6.doi:10.1155/2015/125636. 125636 [PMC free article] [PubMed] [CrossRef] [Google Scholar]

Juneja N, Hegde M. Comparison of the antifungal efficacy of 1.3% NaOCl/MTAD with other routine irrigants: an ex-vivo study. International Scholarly Research Notices. 2014; 2014:5. doi: 10.1155/2014/575748.]

Nevares G, Xavier F, Gominho L, et al. Apical extrusion of debris produced during continuous rotating and reciprocating motion. The Scientific World Journal 2015;2015:5. doi: 10.1155/2015/267264.267264 [PMC free article] [PubMed]

Kini S., Shetty S. V., Shetty K. H., Kudva A., Kumar P. The efficiency of 2.5% sodium hypochlorite in preventing inoculation of periapical tissues with contaminated patency files: an ex vivo evaluation. Journal of Pharmacy and Bioallied Sciences. 2015;7(6): S563–S566. doi: 10.4103/0975-7406.163542.

Mohamed El-Sayed MA, Aziz Taleb AA, Mubarak Balbahaith MS. Sealing ability of three singlecone obturation systems: An invitro glucose leakage study. J Conserv Dent. 2013;16(6):489–493.doi:10.4103/0972-0707.120936

Pirani C, Generali L,Iacono F, Cavani F, Prati C. Evaluation of the root filling quality with experimental carrier-based obturators: A CLSM and FEG-SEM analysis. Aust. Endod. J. 2021, 1–9. [Google Scholar] [CrossRef] [PubMed]

Dasari L, Anwarullah A, Mandava J, Konagala RK, Karumuri S, Chellapilla PK. Influence of obturation technique on penetration depth and adaptation of a bioceramic root canal sealer. J Conserv Dent. 2020 Sep-Oct;23(5):505-511. doi: 10.4103/JCD.JCD_450_20. Epub 2021 Feb 10. PMID: 33911361

Zahid HM, Ghareeb NH, Evaluation of filling ability of Guttaflow Bioseal sealer to the simulated lateral canal by scanning electron microscope: An in vitro study. EDJ Vol.2 No.2 Dec 2019.

Wang Y.Invitro study of dentinal tubule penetration and filling quality of bioceramic sealer. Published online 2018 Feb 1. doi: 10.1371/journal.pone.019224

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Published

2024-01-23

How to Cite

1.
Saeed RK. Evaluation of the penetration depth of Guttaflow bioseal and Bioceramic sealers using scanning electron microscope (in vitro study). EDJ [Internet]. 2024 Jan. 23 [cited 2024 Mar. 5];6(2). Available from: https://edj.hmu.edu.krd/index.php/journal/article/view/248

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