Effects of hyaluronic acid on bone graft healing: An experimental study on sheep
DOI:
https://doi.org/10.15218/edj.2019.15Keywords:
Hyaluronic acid, bone graft, alloplastic material,, guided bone regeneration.Abstract
Background and objectives: Alloplastic is one of known materials have been used to treat bony defects in oral and maxillofacial region, but the healing is much more slower than auto graft . So many interpositional graft materials like hyaluronic acid and bone morphogenetic protein have been introduced to such material to induce osteoinductivity of graft material. The aim was to evaluate the histological effect of Hyaluronic acid on bone graft substitute.
Materials and methods: An experimental study carried out on 3 domestic sheep 2-4 years old. They divided into two groups, first group (study group): bone graft substitute mixed with hyaluronic acid introduced to the created cavities in the basal bone of mandible. Second group (control group): bone graft substitute were introduced into created cavities in the basal bone of mandible and the defects in both groups were covered with collagen membrane. The samples then sacrificed in deferent period interval 2 weeks, 6 weeks and 12 weeks and the sample collected and sent for histological examination.
Results: All animals survived till the end of study. Both groups were characterized by new bone formation in defects however the bone formation in group 1 significantly preceded. Conclusions: Hyaluronic acid induces the onset of new bone formation when mixed with alloplastic material.
Conclusions: Hyaluronic acid induces the onset of new bone formation when mixed with alloplastic material.
References
2. Davis RE, Telischi FF. Traumatic facial nerve injuries: review of diagnosis and treatment. J Craniomaxillofac Trauma. 1995;1(3):30–41.
3. Pape HC, Evans A, Kobbe P. Autologous bone graft: properties and techniques. J Orthop Trauma. 2010 Mar;24 Suppl 1:S36-40. https:// doi.org/10.1097/BOT.0b013e3181cec4a1
4. Zouhary KJ. Bone graft harvesting from distant sites: concepts and techniques. Oral Maxillofac Surg Clin North Am. 2010 Aug;22(3):301–16, v.
5. De Long WG, Einhorn TA, Koval K, McKee M, Smith W, Sanders R, et al. Bone grafts and bone graft substitutes in orthopaedic trauma surgery. A critical analysis. J Bone Joint Surg Am. 2007 Mar;89(3):649–58. https://doi.org/10.2106/ JBJS.F.00465
6. Finkemeier CG. Bone-grafting and bone-graft substitutes. J Bone Joint Surg Am. 2002 Mar;84-A (3):454–64. https://doi.org/10.1111/j.13993089.2012.00694.x
7. Taba M, Jin Q, Sugai JV, Giannobile WV. Currentconcepts in periodontal bioengineering. Orthod Craniofac Res. 2005 Nov;8(4):292–302. https:// doi.org/10.1111/j.1601-6343.2005.00352.x
8. Arpağ OF, Damlar I, Altan A, Tatli U, Günay A, Arpağ OF, et al. To what extent does hyaluronic acid affect healing of xenografts? A histomorphometric study in a rabbit model. Journal of Applied Oral Science [Internet]. 2018 [cited 2018 Oct 31];26. https://doi.org/10.1590/1678-7757-201700049.
9. Bertl K, Bruckmann C, Isberg P-E, Klinge B, Gotfredsen K, Stavropoulos A. Hyaluronan in nonsurgical and surgical periodontal therapy: a systematic review. J Clin Periodontol. 2015 Mar;42(3):236–46. https://doi.org/10.1111/jcpe.12371
10. Zhao N, Wang X, Qin L, Guo Z, Li D. Effect of molecular weight and concentration of hyaluronan on cell proliferation and osteogenic differentiation in vitro. Biochem Biophys Res Commun. 2015 Sep 25;465(3):569–74. https://doi.org/10.1016/ j.bbrc.2015.08.061
11. Chang E-J, Kim HJ, Ha J, Kim HJ, Ryu J, Park K-H, et al. Hyaluronan inhibits osteoclast differentiation via Toll-like receptor 4. Journal of Cell Science. 2007 Jan 1;120(1):166–76. https:// doi.org/10.1242/jcs.03310
12. Zhu H, Mitsuhashi N, Klein A, Barsky LW, Weinberg K, Barr ML, et al. The role of the hyaluronan receptor CD44 in mesenchymal stem cell migration in the extracellular matrix. Stem Cells. 2006 Apr;24(4):928–35. https://doi.org/10.1634/ stemcells.2005-0186.
13. Aslan M, Simsek G, Dayi E. The effect of hyaluronic acid-supplemented bone graft in bone healing: experimental study in rabbits. J Biomater Appl. 2006 Jan;20(3):209–20. https:// doi.org/10.1177/0885328206051047
14. Omar Naser Ihghaf N, Abdel- Monem Tawfik M, Elhawary Y, Ahmed Mansour N. OSTEON II VERSUS BIOGEN IN HEALING OF JAW BONE DEFECTS. Egyptian dental journal. 2015 Jul 1;61:4045:4053.
15. Oryan A, Alidadi S, Moshiri A, Maffulli N. Bone regenerative medicine: classic options, novel strategies, and future directions. J Orthop Surg Res. 2014 Mar 17;9(1):18. https:// doi.org/10.1186/1749-799X-9-18
16. Mescher A. Junqueira’s Basic Histology. 12th Revised edition edition. New York: McGraw-Hill Medical; 2009. 467 p.
17. Fernández RF, Bucchi C, Navarro P, Beltrán V, Borie E. Bone grafts utilized in dentistry: an analysis of patients’ preferences. BMC Med Ethics [Internet]. 2015 Oct 20 [cited 2018 Sep 23];16. https://doi.org/10.1186/s12910-015-0044-6
18. Long B, Dan L, Jian L, Yunyu H, Shu H, Zhi Y. Evaluation of a novel reconstituted bone xenograft using processed bovine cancellous bone in combination with purified bovine bone morphogenetic protein. Xenotransplantation. 2012 Apr;19(2):12–32.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2019 Muhammed A. Mustafa, Othman A. Omar (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright on any article published in Erbil Dental Journal is retained by the author(s) in agreement with the Creative Commons Attribution Non-Commercial ShareAlike License (CC BY-NC-SA 4.0).
