The effect of local propolis irrigation as an adjunct to scaling and root planning on alveolar bone loss in experimental periodontitis in rats (A biochemical and histological study).


  • Khadeeja Mohammed Ali Department of Periodontology, College of Dentistry, Hawler Medical University, Erbil, Iraq.
  • Zewar Ahmad Saleh Department of Periodontology, College of Dentistry, Hawler Medical University, Erbil, Iraq.
  • Jalal Ali Jalal Department of Basic Sciences, College of Medicine, Hawler Medical University, Erbil, Iraq.



Alveolar bone resorption, Alkaline phosphatase enzyme, Ligature induced periodontitis, Propolis


Background and objectives: The present study aimed to investigate the efficacy of local use of propolis (PRO) as adjunctive to scaling and root planing (SRP) in the treatment of alveolar bone loss in rats with ligature induced periodontitis (LIP).
Material and methods: 60 male rats were divided into three groups of 20 rats: the control group (C) with no experimental periodontitis or treatment (gp1); ligature-induced periodontitis treated by SRP with a vehicle irrigation (dimethyl sulfoxide + physiological saline solution) group (gp2); and ligature-induced periodontitis treated by SRP with 10% PRO extract irrigation group (gp3). The irrigation materials were locally applied three times daily for 11 days After removal of the ligature, SRP was performed in gp2 and gp3. Five rats from each group were euthanized on days 0, 7, 14, and 21 after local treatment. Intracardiac blood samples and mandible were obtained for biochemical and histopatholical analysis.
Results: Rats that received local treatment by SRP with propolis irrigation in gp3 revealed features of acceleration of the periodontal tissue-repair process, alveolar bone formation, and significantly higher alkaline phosphatase serum levels (ALP) than gp2 were treated by SRP with vehicle irrigation.
Conclusion: Local use of propolis as an adjunct therapy to SRP can be effective in the treatment of alveolar bone loss in rat model of ligature-induced periodontitis.


Papapanou SN, Sanz M , Buduneli N, DietrichT, Feres M, Fine DH, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri‐Implant Diseases and Conditions. J Periodontol. 2018;89:S173–S82.

Tonetti MS, Greenwell H, Kornman, Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol. 2018; 89:S159–S72.

Martins CS, Leitão RFC, Costa DVS, Melo IM, Santos GS, Lima V, et al. Topical HPMC/SNitrosoglutathione Solution Decreases Inflammation and Bone Resorption in Experimental Periodontal Disease in Rats. PLoS One. 2016;11(4):e0153716. https://10.1371/ journal.pone.0153716.

Hienz SA, Paliwal S, Ivanovski S. Mechanisms of Bone Resorption in Periodontitis. J Immunol Res. 2015;2015:615486. https://10.1155/2015/615486

Akram Z, Safii SH, Vaithilingam RD, Baharuddin NA, Javed F, Vohra F.Efficacy of non-surgical periodontal therapy in the management of chronic periodontitis among obese and non-obese patients: a systematic review and meta-analysis. Clin Oral Inves. 2016;20(5):903–14. https://10.1007/s00784-016-1793-4

Ramesh A, Varghese SS, Doraiswamy JN, Malaiappan S. Herbs as an antioxidant arsenal for periodontal diseases. J Intercult Ethnopharmacol. 2016;5(1):92–6. https://10.5455/jice.20160122065556

Farooqui T, Farooqui AA. Molecular Mechanism Underlying the Therapeutic Activities of Propolis: A Critical Review. Curr Nutr Food Sci. 2010;6:186–99. https://10.2174/157340110792389136

Sforcin JM. and Bankova V. Propolis: Is there a potential for the development of new drugs? J Ethnopharmacol. 2011;133(2):253–60.

Toker H, Ozan F, Ozer H, Ozdemir H, Eren K, Yeler H. A morphometric and histopathologic evaluation of the effects of propolis on alveolar bone loss in experimental periodontitis in rats. J Periodontol. 2008;79(6):1089–94. https://10.1902/jop.2008.070462

Altan BA, Kara IM, Nalcaci R, Ozan F, Erdogan SM, Mahmut MO, et al. Systemic propolis stimulates new bone formation at the expanded suture: a histomorphometric study. Angle Orthod. 2013;83(2):286–91. https://10.2319/032612-253.1

AL-Ani I, Zimmermann S, Reichling J, Wink M . Antimicrobial Activities of European Propolis Collected from Various Geographic Origins Alone and in Combination with Antibiotics. Medicines (Basel). 2018;5(1):2. https://10.3390/medicines5010002

Pimentel SP, Barrella GM, Casarin RCV, Cirano FR, Casati MZ, Foglio MN,et al. Protective effect of topical Cordia verbenacea in a rat periodontitis model: immune-inflammatory, antibacterial and morphometric assays. BMC Complement Altern Med. 2012;12(1):1–8. https://10.1186/1472-6882-12-224

Paixão MS, Melo MS, Damascena NP, Araujo AS, Soares AF, Oliveira DVA, et al. Hyptis pectinata gel prevents alveolar bone resorption in experimental periodontitis in rats. Rev Bras de Farmacogn. 2015;25(1):35–41.

Park S, Kang S, Han C, Kim J, Song C, Lee S, et al. The Effects of Topical Application of Polycal (a 2:98 (g/g) Mixture of Polycan and Calcium Gluconate) on Experimental Periodontitis and Alveolar Bone Loss in Rats. Molecules. 2016;21(4):527. https://10.3390/molecules21040527

Cirano FR, Casarin RCV, Ribeiro FV, Casati MZ, Pimentel SP, Taiete T, et al. Effect of Resveratrol on periodontal pathogens during experimental periodontitis in rats. Braz Oral Res. 2016;30(1):e128. https://10.1590/1807-3107BOR2016.vol30.0128

Lima MR, Lopes AP, Martins C, Brito GAC, Carneiro VC, Goes P. The effect of Calendula officinalis on oxidative stress and bone loss in experimental periodontitis. Front Physiol. 2017;8:440. https://10.3389/fphys.2017.00440

Santos BFE, Souza EQM, Brigagão MRPL, Lima DC, Fernandes LA. Local application of statins in the treatment of experimental periodontal disease in rats. J Appl Oral Sci. 2017;25(2):168–76. https://10.1590/1678-77572016-0149

Aral CA, Kasim S, Greenwell H, Kara K, Cxetin A, Yakan B. Alveolar bone protective and hypoglycemic effects of systemic propolis treatment in experimental periodontitis and diabetes mellitus. J Med Food. 2015;18(2):195–201. https://10.1089/jmf.2013.3137

Cao, Chen, Zhang, You, Wang, And Hu, Mechanisms underlying the wound healing potential of propolis based on its in vitro antioxidant activity. Phytomedicine. 2017;34:76-84. https://10.1016/j.phymed.2017.06.001

Somsanith N, Kim YK, Jang YS, Lee YH, Yi HK, Jang JH, et al. Enhancing of Osseointegration with Propolis-Loaded TiO2 Nanotubes in Rat Mandible for Dental Implants. Materials. 2018;11(1):61. https://10.3390/ma11010061

Pereira YCL, Issa JPM, Watanabe E, Nascimento GC, Iyomasa MM, Ciampo JOD,et al.The Therapeutic Use of Propolis Extract in Alveolar Bone Contaminated with Bacterial Endotoxin. Dentistry. 2018;8(1):1–9. https://10.4172/2161-1122.1000473

de Freitas CV, Galdez LP, Dias HL, Cirelli JA, Souza EM, da Silva VC. Effect of Subgingival Irrigation with Different Substances in the Treatment of Periodontal Disease. A Histometric Study in Rats. J Int Acad Periodontol. 2016;18(1):2–6.

Nakajima M, Arimatsu K, Minagawa T, Matsuda Y, Sato S, Takahashi N, et al. Brazilian propolis mitigates impaired glucose and lipid metabolism in experimental periodontitis in mice. BMC Complement Altern Med. 2016;16(1):329.


Braakhuis A. Evidence on the Health Benefits of Supplemental Propolis. Nutrients. 2019;11(11):2705. https://10.3390/nu11112705

Kresnoadi U, Rahayu RP, Ariani MD, Soesanto A, The Potential of Natural Propolis Extract Combined with Bovine Bone Graft in Increasing Heat Shock Protein 70 and Osteocalcin on Socket Preservation. Eur J Dent. 2020;14(1):31–7. https://10.1055/s-0040-1701921

Kresnoadi U, Rahmania PN, Caesar HU, Djulaeha E, Agustono B, Ari M. The role of the combination of Moringa oleifera leaf extract and demineralized freeze-dried bovine bone xenograft (xenograft) as tooth extraction socket preservation materials on osteocalcin and transforming growth factor-beta 1 expressions in alveolar bone of Cavia cobaya. J Indian Prosthodont Soc. 2019.19(2):120–5. https://10.4103/jips.jips_251_18

Lunardhi LC, Kresnoadi UK, Agustono B. The effect of a combination of propolis extract and bovine bone graft on the quantity of fibroblasts, osteoblasts and osteoclasts in tooth extraction sockets. Dent J. 2019;52:126–32. https://10.20473/j.djmkg.v52.i3.p126-132

Delgado-Calle D, Sañudo C, Sánchez-Verde L, García-Renedo RJ, Arozamena J, Riancho JA. Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage. Bone. 2011;49(4):830–8. https://10.1016/j.bone.2011.06.006

Cetinkaya BO, Acikcoz G, Keles GC, Ayas B, Korkmaz A. The Effect of Cyclosporin A on Alveolar Bone in Rats Subjected to Experimental Periodontal Disease. Toxicol Pathol. 2006.34(6):716–22. https://10.1080/01926230600826269

Wu YH, Taya Y, Kuraji R, Ito H, Soeno Y, Numabe Y. Dynamic microstructural changes in alveolar bone in ligature-induced experimental periodontitis. Odontology. 2020;108(3):339–49. https://10.1007/s10266-019-00471-1

Chen J, Deng L, Porter C, Alexander G, Patel D, Vines J, et al. Angiogenic and Osteogenic Synergy of Human Mesenchymal Stem Cells and Human Umbilical Vein Endothelial Cells Cocultured on a Nanomatrix. Sci Rep. 2018;8(1):15749. https://10.1038/s41598-018-34033-2.




How to Cite

Ali KM, Saleh ZA, Jalal JA. The effect of local propolis irrigation as an adjunct to scaling and root planning on alveolar bone loss in experimental periodontitis in rats (A biochemical and histological study). EDJ [Internet]. 2022 Jul. 30 [cited 2023 Jun. 4];5(1):101-9. Available from:



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